IVErigidprep Banner

Protocol – InVitro Express Rigid Vessel

rigidvesselconfigOASIS® Grower Solutions Tissue Culture Growing Medium

Achieve superior in vitro root development, rapid handling at transplant and faster greenhouse growth

OASIS® InVitro Express (IVE) for rigid vessel comes in a sheet with 30 or 35 cells. The cells are separated by grooves running the length and width of sheet. Each cell has a star shaped universal dibble hole that can accept very thin to thick caliper micro cuttings.

  • Sheet dimensions of 30 count and 35 count: 2.775″ (7.05 cm) x 3.575″ (9.08 cm) x 1″ (2.54 cm).
  • Each sheet of 30 count/35 count can hold up to 120 ml of media.
  • Cell dimensions for 30 count sheet: 0.511″ (1.30 cm) x 0.550″ (1.40) x 1″ (2.54 cm).
  • Cell dimensions for 35 count sheet: 0.50″ (1.27 cm) x 0.46″ (1.17 cm) x 1″ (2.54 cm)
  • Rigid Vessel RV-750 dimensions: 3.54″ (9cm) x 4.25″ (10.8) x 3.40 (9.9 cm)

General Instructions for using OASIS® InVitro Express in a rigid vessel for In Vitro Rooting (stage III)

Media Preparation

Place the IVE sheet in RV-750. Dispense the medium with or without gelling agent in each vessel.

IVErigidprep

Media Volume

  • If you are using this matrix for the first time on a new crop, it is highly recommended to conduct a media volume screening experiment to identify optimal media for your specific crop. The lower the media volume used, the higher the air-filled pore space of the matrix
  • For initial screening, consider 100% (120 ml/sheet), 85 (100 ml/sheet), and 75% (90 ml/sheet) media holding capacity.

Note: Several crops are responding positively to liquid culture while some are not responding. This is possibly due to the media composition or the liquid media itself. For this reason we are recommending to run the first set of screening experiments without and also with gelling agent to better understand the optimal culture media.

Autoclaving and Initiating CultureIVEautoclav
  • For details about experimental treatments for initial screening, refer to experimental design sheet. After adding media with or without gelling agent, place the lids and autoclave using a standard cycle with slow, fluid exhaust. The autoclaving process allows the liquid media to move into the cellular foam.

Note: If it is not possible to add culture medium before the autoclaving process, first autoclave the IVE sheets dry. Place the autoclaved IVE sheet in the vessel and add hot (~100° C) sterile media without or with gelling agent over the top. Adding hot media allows it to move into the cellular foam. Make sure the foam is saturated without any dry spots. Vessels can be stored after cooling by enclosing the entire tray in a sterile, plastic bag.grooves. Up pot the plantlet with foam and roots intact.

IVEtransplantprep

  • When ready to start the culture under hood, remove the lid, and insert the shoots into the dibble hole.
  • Insert the cuttings approximately 1 – 1.25 cm deep so there is sufficient area in the cube to promote uniform rooting. Note: inserting cuttings too deep can result in compromised oxygen availability and slow down the root development.
  • Place the lid and seal it with a tape.
  • Adjust the vent as required and follow the standard culture conditions.

For transplant

  • When the plant material is ready for transplant, open the lid and tilt the vessel sideways to remove the entire IVE matrix with the rooted plantlets into a standard greenhouse tray with drain holes.
  • Place the tray on a greenhouse bench and flush the matrix with greenhouse fertilizer, using a hose and breaker. This process allows medium and incorporating fertilizer in one step.
  • Separate the cubes by breaking the foam along the
  • Acclimatize the plants as required. The acclimatization will be faster and easier since the plantlets will have a stronger root system and minimal damage during transplant.

 

FAQ

Q. Are the vessels and the filter membrane reusable?

A. Yes both vessels and filter membrane are reusable. If there is any mechanical damage, then the filter membranes needs to be replaced.

Q. Can I place the shoots on the surface of the IVE growing medium without inserting them?

A. No, the shoots should be inserted into the matrix so the cut end of the shoot is exposed to the liquid media.

Q. How should I store the foam IVE growing medium?

A. The foam matrix can be stored at room temperature in a closed carton. During storage the foam should not be exposed to light for a prolonged period of time because the UV light can make the foam brittle.

Q. How long can I store the foam matrix?

A. When stored properly as explained above, the foam matrix can be stored for more than a year.

Stay up to date with all of the latest products, upcoming events & much more.
SUBSCRIBE
Copyright © 2016 OASIS® Grower Solutions. Privacy. Web design by Mobkii